creators_name: Wangoo, Nishima
creators_name: Bhasin, K K
creators_name: Boro, Robin C
creators_name: Suri, C Raman
type: article
datestamp: 2011-11-30 09:36:13
lastmod: 2012-03-29 06:16:08
metadata_visibility: show
title: Facile synthesis and functionalization of water-soluble gold nanoparticles for a bioprobe.
ispublished: pub
subjects: QD
full_text_status: restricted
keywords: Amine-capped gold nanoparticles; Octadecylamine; Bioconjugation; Binding constants
note: Copyright of this article belongs to Elsevier Science
abstract: In this work, we report the size tunable synthesis of water-dispersed gold nanoparticles by using octadecylamine (ODA) as the reducing agent, that electrostatically complexes with the chloroaurate ions, reduces them, and subsequently caps the gold nanoparticles. Amine-capped gold nanoparticles, thus formed, were subsequently coordinated with a secondary monolayer of an anionic surfactant, sodium bis(2-ethylhexyl)-sulfosuccinate (AOT) which helps in providing sufficient hydrophilicity to the gold nanoparticles. Functionalized gold nanoparticles were characterized by UV-vis, IR spectrophotometric, dynamic light scattering, zeta-potential and transmission electron microscopic techniques, which demonstrated high stability of gold nanoparticles in aqueous media, indicating stabilization via bilayers of ODA and AOT. The gold nanoparticles were further conjugated with a protein (bovine serum albumin) and the interaction was investigated by circular dichroism studies as well as by measuring the fluorescence quenching of the tryptophan residues of protein molecules after the binding of nanoparticles to specific sites of the protein. The binding constant and the stoichiometry values indicated that the particles with larger core size are less site-specific but show higher binding affinity with protein molecules. The use of a bio-compatible synthetic process and the stabilization of the gold nanoparticles by ODA and AOT are interesting from the point of view of making bioprobes for life science applications.
date: 2008-03-03
date_type: published
publication: Analytica chimica acta
volume: 610
number: 1
publisher: Elsevier Science
pagerange: 142-8
refereed: TRUE
issn: 1873-4324
official_url: http://www.sciencedirect.com/science/article/pii/S0003267008000901
related_url_url: http://www.sciencedirect.com/science/article/pii/S0003267008000901
related_url_type: pub
citation:   Wangoo, Nishima and Bhasin, K K and Boro, Robin C and Suri, C Raman  (2008) Facile synthesis and functionalization of water-soluble gold nanoparticles for a bioprobe.  Analytica chimica acta, 610 (1).  pp. 142-8.  ISSN 1873-4324     
document_url: http://crdd.osdd.net/open/615/1/suri08.1.pdf