TY  - JOUR
N1  - Copyright of this article belongs to Biotechniques.com
ID  - open616
UR  - http://www.biotechniques.com/multimedia/archive/00003/BTN_A_000112671_O_3569a.pdf
IS  - 1
A1  - Ahmed, Shakil
A1  - Singh, Jagmohan
Y1  - 2008/01//
N2  - Here we describe a method that allows selective detection of silent copy transcripts in homothallic strains of Schizosaccharomyces pombe in the presence of the active cassettes. The method involving RT-PCR (reverse transcriptase polymerase chain reaction) exploits our observation that the silent copy transcripts extend beyond the regions of homology to the flanking sequences specific for the donor cassettes, thus allowing design of oligos that are specific for the different donors. The results are validated using a known silencing mutant swi6.
PB  - Informa BioSciences
JF  - BioTechniques
VL  - 44
SN  - 0736-6205
TI  - RT-PCR method for selective detection of silent gene transcripts in silencing mutants in homothallic strains of Schizosaccharomyces pombe.
AV  - restricted
ER  -