creators_name: Ahmed, Shakil
creators_name: Singh, Jagmohan
type: article
datestamp: 2011-11-30 09:25:54
lastmod: 2011-11-30 09:26:21
metadata_visibility: show
title: RT-PCR method for selective detection of silent gene transcripts in silencing mutants in homothallic strains of Schizosaccharomyces pombe.
ispublished: pub
subjects: QH301
full_text_status: restricted
note: Copyright of this article belongs to Biotechniques.com
abstract: Here we describe a method that allows selective detection of silent copy transcripts in homothallic strains of Schizosaccharomyces pombe in the presence of the active cassettes. The method involving RT-PCR (reverse transcriptase polymerase chain reaction) exploits our observation that the silent copy transcripts extend beyond the regions of homology to the flanking sequences specific for the donor cassettes, thus allowing design of oligos that are specific for the different donors. The results are validated using a known silencing mutant swi6.
date: 2008-01
date_type: published
publication: BioTechniques
volume: 44
number: 1
publisher: Informa BioSciences
pagerange: 54, 56, 58
refereed: TRUE
issn: 0736-6205
official_url: http://www.biotechniques.com/multimedia/archive/00003/BTN_A_000112671_O_3569a.pdf
related_url_url: http://www.biotechniques.com/multimedia/archive/00003/BTN_A_000112671_O_3569a.pdf
related_url_type: pub
citation:   Ahmed, Shakil and Singh, Jagmohan  (2008) RT-PCR method for selective detection of silent gene transcripts in silencing mutants in homothallic strains of Schizosaccharomyces pombe.  BioTechniques, 44 (1).  54, 56, 58.  ISSN 0736-6205     
document_url: http://crdd.osdd.net/open/616/1/jagmohan08.pdf