<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Inhibition of bfl-1/A1 by siRNA inhibits mycobacterial growth in THP-1 cells by enhancing phagosomal acidification."^^ . "Virulent tubercle bacilli inhibit apoptosis to establish a safe environment within the host cells. Here, we report that NF-kappaB dependent antiapoptotic protein bfl-1/A1 plays an important role in this process. Both virulent and avirulent mycobacteria bearing THP-1 cells expressed considerable amount of bfl-1/A1 after 4 h of infection. However, after 48 h of infection, bfl-1/A1 expression was evident only in Mycobacterium tuberculosis H37Rv but not in M. tuberculosis H37Ra infected cells. When parallel experiments were performed with Human monocyte-derived macrophages (MDMs), differential expression of bfl-1/A1 mRNA was observed in case of M. tuberculosis H37Rv and M. tuberculosis H37Ra infection. siRNA mediated inhibition of bfl-1/A1 induced apoptosis in M. tuberculosis H37Rv infected THP-1 and MDMs. Reduction in intracellular mycobacterial growth was observed in bfl-1/A1 siRNA transfected, M. tuberculosis H37Rv infected THP-1 cells. Enhancement of phagosome-lysosome fusion was observed in bfl-1/A1 siRNA treated and M. tuberculosis H37Rv infected THP-1 cells. These results clearly indicated that differential expression of bfl-1/A1 in M. tuberculosis H37Rv and M. tuberculosis H37Ra infected THP-1 cells probably account for the difference in infection outcome."^^ . "2008-04" . . "1780" . "4" . . "Elsevier Science"^^ . . . "Biochimica et biophysica acta"^^ . . . "00063002" . . . . . . . . . . . . . . . . "Manoj"^^ . "Raje"^^ . "Manoj Raje"^^ . . "Mahesh"^^ . "Kathania"^^ . "Mahesh Kathania"^^ . . "Sekhar"^^ . "Majumdar"^^ . "Sekhar Majumdar"^^ . . "Rohan"^^ . "Dhiman"^^ . "Rohan Dhiman"^^ . . . . . . "Inhibition of bfl-1/A1 by siRNA inhibits mycobacterial growth in THP-1 cells by enhancing phagosomal acidification. (PDF)"^^ . . . . . "Inhibition of bfl-1/A1 by siRNA inhibits mycobacterial growth in THP-1 cells by enhancing phagosomal acidification. (Indexer Terms)"^^ . . . . . . "Inhibition of bfl-1/A1 by siRNA inhibits mycobacterial growth in THP-1 cells by enhancing phagosomal acidification. (Image (PNG))"^^ . . . . . . "Inhibition of bfl-1/A1 by siRNA inhibits mycobacterial growth in THP-1 cells by enhancing phagosomal acidification. (Image (PNG))"^^ . . . . . . "Inhibition of bfl-1/A1 by siRNA inhibits mycobacterial growth in THP-1 cells by enhancing phagosomal acidification. (Image (PNG))"^^ . . . . . . "Inhibition of bfl-1/A1 by siRNA inhibits mycobacterial growth in THP-1 cells by enhancing phagosomal acidification. (Image (PNG))"^^ . . . . . "HTML Summary of #617 \n\nInhibition of bfl-1/A1 by siRNA inhibits mycobacterial growth in THP-1 cells by enhancing phagosomal acidification.\n\n" . "text/html" . . . "QD Chemistry"@en . .