@article{open664,
          volume = {29},
          number = {1-2},
          author = {U C Banerjee},
            note = {Copyright of this article belongs to Elsevier Science.},
           title = {Transformation of rifamycin B with soluble rifamycin oxidase from Curvularia lunata},
       publisher = {Elsevier Science},
            year = {1993},
         journal = {Journal of Biotechnology},
           pages = {137--143},
        keywords = {Curvularia lunata; Rifamycin B; Soluble rifamycin oxidase; Optimization},
             url = {http://crdd.osdd.net/open/664/},
        abstract = {Transformation of rifamycin B to rifamycin S using soluble rifamycin oxidase from Curvularia lunata was carried out in shake flask and in a reactor. Optimum transformation temperature was found to be at 50?C but, from the practical point of view, all the transformation reactions were carried out at 28?C. In non-stirring condition it took 50 h to transform the 1\% (w/v) solution of rifamycin B, while in stirring condition (200 rpm) it took only 3 h. Five percent enzyme solution (activity 11 IU ml?1) was found to be optimum for the transformation reaction. An aeration rate of 1?1.5 vvm was maintained during transformation reaction in a batch reactor. With higher substrate concentration (3\%, w/v) it took more time (7 h) to complete the transformation than with 1\% (w/v) rifamycin B (3 h).

A few drops of antifoam (polypropylene glycol) was effective in suppressing the foam formed during aeration in the reactor and it did not interfere in the isolation of rifamycin S.
}
}