creators_name: Banerjee, U C
type: article
datestamp: 2012-01-16 15:21:13
lastmod: 2012-03-28 10:01:30
metadata_visibility: show
title: Transformation of rifamycin B with soluble rifamycin oxidase from Curvularia lunata
ispublished: pub
subjects: QR
full_text_status: none
keywords: Curvularia lunata; Rifamycin B; Soluble rifamycin oxidase; Optimization
note: Copyright of this article belongs to Elsevier Science.
abstract: Transformation of rifamycin B to rifamycin S using soluble rifamycin oxidase from Curvularia lunata was carried out in shake flask and in a reactor. Optimum transformation temperature was found to be at 50°C but, from the practical point of view, all the transformation reactions were carried out at 28°C. In non-stirring condition it took 50 h to transform the 1% (w/v) solution of rifamycin B, while in stirring condition (200 rpm) it took only 3 h. Five percent enzyme solution (activity 11 IU ml−1) was found to be optimum for the transformation reaction. An aeration rate of 1–1.5 vvm was maintained during transformation reaction in a batch reactor. With higher substrate concentration (3%, w/v) it took more time (7 h) to complete the transformation than with 1% (w/v) rifamycin B (3 h).

A few drops of antifoam (polypropylene glycol) was effective in suppressing the foam formed during aeration in the reactor and it did not interfere in the isolation of rifamycin S.

date: 1993
publication: Journal of Biotechnology
volume: 29
number: 1-2
publisher: Elsevier Science
pagerange: 137-143
id_number: doi:10.1016/0168-1656(93)90046-P
refereed: TRUE
issn: 01681656
official_url: http://dx.doi.org/10.1016/0168-1656(93)90046-P
citation:   Banerjee, U C  (1993) Transformation of rifamycin B with soluble rifamycin oxidase from Curvularia lunata.  Journal of Biotechnology, 29 (1-2).  pp. 137-143.  ISSN 01681656