title: Biophysical characterization of Vibrio El Tor typing phage e5.
creator: Basu, R
creator: Ghosh, A N
creator: Dasgupta, S
creator: Ghosh, A
subject: QR Microbiology
description: Vibrio cholerae typing phage e5, which can lyse only the El Tor strains of V. cholerae, was characterized. The phage had a polyhedral head 51 nm in diameter and a short tail 13 nm in length. It contained 13 structural polypeptides, with the molecular mass of the major component being 50 kDa. Phage chromosome comprised a 38.5-kb linear double-stranded DNA molecule with unique termini, as determined by restriction fragment analysis and electron microscopy, and had a G+C content of 35.5%. A physical map was constructed with the restriction endonucleases HaeII and HpaII. Adsorption of the phage to its host followed a biphasic kinetics and its intracellular growth was characterized by a latent period of 15 min and a burst size of 100 particles per infected cell. The phage was found to be moderately thermotolerant.
publisher: Wiley
date: 1993-01-01
type: Article
type: PeerReviewed
format: application/pdf
identifier: http://crdd.osdd.net/open/674/1/Ghosh1993.pdf
identifier:   Basu, R and Ghosh, A N and Dasgupta, S and Ghosh, A  (1993) Biophysical characterization of Vibrio El Tor typing phage e5.  FEMS microbiology letters, 106 (1).  pp. 9-15.  ISSN 0378-1097     
relation: http://crdd.osdd.net/open/674/