creators_name: Basu, R
creators_name: Ghosh, A N
creators_name: Dasgupta, S
creators_name: Ghosh, A
type: article
datestamp: 2012-01-16 15:23:39
lastmod: 2014-03-27 09:19:55
metadata_visibility: show
title: Biophysical characterization of Vibrio El Tor typing phage e5.
ispublished: pub
subjects: QR
full_text_status: none
note: Copyright of this article belongs to Wiley.
abstract: Vibrio cholerae typing phage e5, which can lyse only the El Tor strains of V. cholerae, was characterized. The phage had a polyhedral head 51 nm in diameter and a short tail 13 nm in length. It contained 13 structural polypeptides, with the molecular mass of the major component being 50 kDa. Phage chromosome comprised a 38.5-kb linear double-stranded DNA molecule with unique termini, as determined by restriction fragment analysis and electron microscopy, and had a G+C content of 35.5%. A physical map was constructed with the restriction endonucleases HaeII and HpaII. Adsorption of the phage to its host followed a biphasic kinetics and its intracellular growth was characterized by a latent period of 15 min and a burst size of 100 particles per infected cell. The phage was found to be moderately thermotolerant.
date: 1993-01-01
date_type: published
publication: FEMS microbiology letters
volume: 106
number: 1
publisher: Wiley
pagerange: 9-15
refereed: TRUE
issn: 0378-1097
citation:   Basu, R and Ghosh, A N and Dasgupta, S and Ghosh, A  (1993) Biophysical characterization of Vibrio El Tor typing phage e5.  FEMS microbiology letters, 106 (1).  pp. 9-15.  ISSN 0378-1097     
document_url: http://crdd.osdd.net/open/674/1/Ghosh1993.pdf