title: Sensitivity Of Recombinant Fermentation With Run-Away Plasmids: A Structured Analysis Of The Effect Of Dilution Rate.
creator: PATNAIK, P.R.
subject: QD Chemistry
description: A recently validated four-compartment model is used to describe intra-cellular dynamics during fermentations with recombinant microorganisms containing temperature-sensitive (run-away) plasmids. The time-dependent sensitivities with respect to the dilution rate have been computed for normal and run-away replication. During normal replication the cells are most sensitive in the early stage of fermentation, and the intra-cellular compartment containing RNA and ribosomes is more sensitive than the other three lumped components. This is explained through a buffering effect. During run-away replication the sensitives are either constant or increase slowly with time, and there is a shift in the relative sensitivities of the intra-cellular components. All sensitivities except that of the substrate increase as the dilution rate increases.
publisher: Taylor and francis group
date: 1995
type: Article
type: PeerReviewed
format: application/pdf
identifier: http://crdd.osdd.net/open/699/1/pat95%28s%29.pdf
relation: http://dx.doi.org/10.1080/00986449508936287
identifier:   PATNAIK, P.R.  (1995) Sensitivity Of Recombinant Fermentation With Run-Away Plasmids: A Structured Analysis Of The Effect Of Dilution Rate.  Chemical Engineering Communications, 131 (1).  pp. 125-140.  ISSN 0098-6445     
relation: http://crdd.osdd.net/open/699/