…
comment: The repository administrator has not yet configured an RDF license.
primary topic: Disruption of a recombinant yeast for the release of beta-galactosidase.

Disruption of a recombinant yeast for the release of beta-galactosidase.
type: AcademicArticle
type: Article
abstract: A recombinant yeast, Saccharomyces cerevisiae, expressing Escherichia coli beta-galactosidase gene under the control of CYC1 constitutive promoter of the yeast, was disrupted in a continuous flow, high speed, bead mill for the release of intracellular beta-galactosidase (EC 3.2.1.23). Release of the beta-galactosidase activity was characterized with respect to glass bead loading in the grinding chamber (70-85% of chamber volume), diameter of the beads (0.25-0.75 mm), number of passes of the cell slurry through the mill (0-6 passes), flow rate of the slurry (25-250 mL.min-1), cell concentration in the slurry (5-20 gDW.L-1), the agitation rotor speed (1000-4000 rpm) and the pH of the slurry (pH 5-10). The optimal conditions for the release of the enzyme were pH 6.0-9.0, 85% loading of 0.5 mm diameter beads and an agitation speed of 2000 rpm. The enzyme release followed first-order kinetics. For otherwise fixed conditions, the extent of cell disruption increase with increasing bead load, number of passes and agitation rotor speed. Cell concentration did not affect disruption. The release of beta-galactosidase activity declined with increasing flow rate of the cell slurry through the mill, but the disruption rate constant increased with flow rate. Under optimal condition, three passes through the grinding chamber were sufficient to release all of the enzyme. In comparison with disruption in the bead mill, chloroform-sodium dodecyl sulfate induced lysis of cells was ineffective in releasing the enzyme quantitatively.
date: 1994-10
volume: 4
issue: 5
is part of: Bioseparation
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
has document: Disruption of a recombinant yeast for the release of beta-galactosidase. (PDF)
has version: Disruption of a recombinant yeast for the release of beta-galactosidase. (PDF)
has published: Disruption of a recombinant yeast for the release of beta-galactosidase. (PDF)
has document: Disruption of a recombinant yeast for the release of beta-galactosidase. (Image (PNG))
subject: QR Microbiology

Disruption of a recombinant yeast for the release of beta-galactosidase. (PDF)
type: Document

Kluwer Academic Publishers
type: Organization

Bioseparation
type: Collection
issn: 0923179X

U C Banerjee
type: Person
given name: U C
family name: Banerjee

M. Moo-Young
type: Person
given name: M.
family name: Moo-Young

Y. Chisti
type: Person
given name: Y.
family name: Chisti

F Garrido
type: Person
given name: F
family name: Garrido

Disruption of a recombinant yeast for the release of beta-galactosidase. (Image (PNG))
type: Document
is version of: Disruption of a recombinant yeast for the release of beta-galactosidase. (PDF)
is volatile version of: Disruption of a recombinant yeast for the release of beta-galactosidase. (PDF)
islightbox thumbnail version of: Disruption of a recombinant yeast for the release of beta-galactosidase. (PDF)

Disruption of a recombinant yeast for the release of beta-galactosidase. (Image (PNG))
type: Document
is version of: Disruption of a recombinant yeast for the release of beta-galactosidase. (PDF)
is volatile version of: Disruption of a recombinant yeast for the release of beta-galactosidase. (PDF)
ispreview thumbnail version of: Disruption of a recombinant yeast for the release of beta-galactosidase. (PDF)

Disruption of a recombinant yeast for the release of beta-galactosidase. (Image (PNG))
type: Document
is version of: Disruption of a recombinant yeast for the release of beta-galactosidase. (PDF)
is volatile version of: Disruption of a recombinant yeast for the release of beta-galactosidase. (PDF)
ismedium thumbnail version of: Disruption of a recombinant yeast for the release of beta-galactosidase. (PDF)

Disruption of a recombinant yeast for the release of beta-galactosidase. (Image (PNG))
type: Document
is version of: Disruption of a recombinant yeast for the release of beta-galactosidase. (PDF)
is volatile version of: Disruption of a recombinant yeast for the release of beta-galactosidase. (PDF)
issmall thumbnail version of: Disruption of a recombinant yeast for the release of beta-galactosidase. (PDF)

HTML Summary of #708 Disruption of a recombinant yeast for the release of beta-galactosidase.
format: text/html
primary topic: Disruption of a recombinant yeast for the release of beta-galactosidase.

QR Microbiology
type: Concept