creators_name: Kar, S
creators_name: Ghosh, R K
creators_name: Ghosh, A N
creators_name: Ghosh, A
type: article
datestamp: 2012-01-27 15:29:51
lastmod: 2012-01-27 15:29:51
metadata_visibility: show
title: Integration of the DNA of a novel filamentous bacteriophage VSK from Vibrio cholerae 0139 into the host chromosomal DNA.
ispublished: pub
subjects: QR
full_text_status: none
keywords: Vibrio cholerae 0139;Filamentous phage;Single-stranded DNA phage;Integration
note: Copyright of this article belongs to Wiley.
abstract: An unusual filamentous bacteriophage, VSK, containing single-stranded, circular DNA as its genome was isolated from Vibrio cholerae 0139 strains P07 and B04. Unlike other single-stranded DNA phages, VSK can integrate its genome into the chromosome of the host and enter into a lysogenic state. The double-stranded replicative form (RF) of the single-stranded phage DNA was isolated. A restriction map of the VSK RF DNA was constructed using HaeII, AvaII, ClaI and XbaI. By Southern blot analysis of the chromosomal DNA of the lysogen using labeled phage DNA as probe, the attachment site (attP) on the viral genome was also identified.
date: 1996-11-15
date_type: published
publication: FEMS microbiology letters
volume: 145
number: 1
publisher: Wiley
pagerange: 17-22
refereed: TRUE
issn: 0378-1097
official_url: http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.1996.tb08550.x/abstract
related_url_url: http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.1996.tb08550.x/abstract
related_url_type: pub
citation:   Kar, S and Ghosh, R K and Ghosh, A N and Ghosh, A  (1996) Integration of the DNA of a novel filamentous bacteriophage VSK from Vibrio cholerae 0139 into the host chromosomal DNA.  FEMS microbiology letters, 145 (1).  pp. 17-22.  ISSN 0378-1097