@article{open737, volume = {21}, number = {6}, author = {B.R. Mohapatra and R K Sani and U C Banerjee}, note = {Copyright of this article belongs to Wiley.}, title = {Characterization of L-asparaginase from Bacillus sp. isolated from an intertidal marine alga (Sargassum sp.)}, publisher = {Wiley}, year = {1995}, journal = {Letters in Applied Microbiology}, pages = {380--383}, url = {http://crdd.osdd.net/open/737/}, abstract = {The bacterial flora associated with an intertidal marine alga (Sargassum sp.) were screened for the presence of extracellular L-asparaginase; one out of five Bacillus strains was found positive. The maximum L-asparaginase activity was found at 37?C and pH 8.0. The optimum NaCl concentration for enzyme activity was found to be 2\% (w/v). The enzyme activity was not affected by the addition of different metal ions (Ca2+, Co2+, Fe2+, Mg2+and Ni2+) at 10 mmol 1-1, but was strongly inhibited by EDTA.} }