%0 Journal Article %@ 0266-8254 %A Mohapatra, B.R. %A Sani, R K %A Banerjee, U C %D 1995 %F open:737 %I Wiley %J Letters in Applied Microbiology %N 6 %P 380-383 %T Characterization of L-asparaginase from Bacillus sp. isolated from an intertidal marine alga (Sargassum sp.) %U http://crdd.osdd.net/open/737/ %V 21 %X The bacterial flora associated with an intertidal marine alga (Sargassum sp.) were screened for the presence of extracellular L-asparaginase; one out of five Bacillus strains was found positive. The maximum L-asparaginase activity was found at 37°C and pH 8.0. The optimum NaCl concentration for enzyme activity was found to be 2% (w/v). The enzyme activity was not affected by the addition of different metal ions (Ca2+, Co2+, Fe2+, Mg2+and Ni2+) at 10 mmol 1-1, but was strongly inhibited by EDTA. %Z Copyright of this article belongs to Wiley.