title: Biosurfactant production by a thermophilic Bacillus subtilis strain. creator: Makkar, R S creator: Cameotra, Swaranjit Singh subject: QR Microbiology description: A strain of Bacillus subtilis was able to grow and produce a biosurfactant on 2% sucrose at 45°C. As a result of biosurfactant synthesis the surface tension of the medium was reduced from 68 dynes cm−1 to 28 dynes cm−1. The strain had the capacity to produce the biosurfactant at high NaCl concentrations (4%) and a wide range of pH (4.5–10.5). The biosurfactant retained its surface-active properties after heating at 100°C for 2 h and at different pH values (4.5–10.5). A maximum amount of biosurfactant was produced when urea or nitrate ions were supplied as nitrogen source. The use of the biosurfactant at high temperatures, acidic, alkaline and saline environments is discussed. As a result of its action, 62% of oil in a sand pack column could be recovered, indicating its potential application in microbiologically enhanced oil recovery. publisher: Springer Science date: 1997 type: Article type: PeerReviewed format: application/pdf identifier: http://crdd.osdd.net/open/769/1/swaranjit1997.1.pdf relation: http://dx.doi.org/10.1038/sj.jim.2900349 identifier: Makkar, R S and Cameotra, Swaranjit Singh (1997) Biosurfactant production by a thermophilic Bacillus subtilis strain. Journal of Industrial Microbiology and Biotechnology, 18 (1). pp. 37-42. ISSN 1367-5435 relation: http://crdd.osdd.net/open/769/