%0 Journal Article
%@ 01410229
%A Sidhu, G.S.
%A Sharma, P
%A Chakrabarti, T
%A Gupta, J.K.
%D 1997
%F open:772
%I Elsevier Science
%J Enzyme and Microbial Technology
%K Thermoamylase; mutagenesis; cloning; subcloning; plasmid stability
%N 7
%P 525-530
%T Strain improvement for the production of a thermostable α-amylase
%U http://crdd.osdd.net/open/772/
%V 21
%X Bacillus sp. MK716, a thermophilic and amylolytic soil isolate, produced a thermostable α-amylase active even at 100°C. The enzyme yield was increased 40 times through mutagenesis with ethylmethane sulfonate. The mutated gene was cloned in Escherichia coli-pBR322 system, and the 4.8 kb cloned fragment was mapped with restriction enzymes. Subcloning in Bacillus subtilis of a 2.0 kb portion of the cloned fragment resulted in 107 times increased enzyme yield. The recombinant plasmid was completely stable in B. subtilis.
%Z Copyright of this article belongs to Elsevier Science.