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primary topic: Strain improvement for the production of a thermostable α-amylase

Strain improvement for the production of a thermostable α-amylase
type: AcademicArticle
type: Article
abstract: Bacillus sp. MK716, a thermophilic and amylolytic soil isolate, produced a thermostable α-amylase active even at 100°C. The enzyme yield was increased 40 times through mutagenesis with ethylmethane sulfonate. The mutated gene was cloned in Escherichia coli-pBR322 system, and the 4.8 kb cloned fragment was mapped with restriction enzymes. Subcloning in Bacillus subtilis of a 2.0 kb portion of the cloned fragment resulted in 107 times increased enzyme yield. The recombinant plasmid was completely stable in B. subtilis.
date: 1997
volume: 21
issue: 7
is part of: Enzyme and Microbial Technology
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
has document: Strain improvement for the production of a thermostable α-amylase (PDF)
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has published: Strain improvement for the production of a thermostable α-amylase (PDF)
has document: Strain improvement for the production of a thermostable α-amylase (Image (PNG))
has document: Strain improvement for the production of a thermostable α-amylase (Indexer Terms)
subject: QR Microbiology

Strain improvement for the production of a thermostable α-amylase (PDF)
type: Document

Elsevier Science
type: Organization

Enzyme and Microbial Technology
type: Collection
issn: 01410229

J.K. Gupta
type: Person
given name: J.K.
family name: Gupta

T Chakrabarti
type: Person
given name: T
family name: Chakrabarti

P Sharma
type: Person
given name: P
family name: Sharma

G.S. Sidhu
type: Person
given name: G.S.
family name: Sidhu

Strain improvement for the production of a thermostable α-amylase (Image (PNG))
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Strain improvement for the production of a thermostable α-amylase (Image (PNG))
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Strain improvement for the production of a thermostable α-amylase (Image (PNG))
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Strain improvement for the production of a thermostable α-amylase (Indexer Terms)
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primary topic: Strain improvement for the production of a thermostable α-amylase

QR Microbiology
type: Concept