TY  - JOUR
ID  - open772
UR  - http://dx.doi.org/10.1016/S0141-0229(97)00055-0
IS  - 7
A1  - Sidhu, G.S.
A1  - Sharma, P
A1  - Chakrabarti, T
A1  - Gupta, J.K.
N2  - Bacillus sp. MK716, a thermophilic and amylolytic soil isolate, produced a thermostable ?-amylase active even at 100°C. The enzyme yield was increased 40 times through mutagenesis with ethylmethane sulfonate. The mutated gene was cloned in Escherichia coli-pBR322 system, and the 4.8 kb cloned fragment was mapped with restriction enzymes. Subcloning in Bacillus subtilis of a 2.0 kb portion of the cloned fragment resulted in 107 times increased enzyme yield. The recombinant plasmid was completely stable in B. subtilis.
VL  - 21
TI  - Strain improvement for the production of a thermostable ?-amylase
AV  - restricted
EP  - 530
N1  - Copyright of this article belongs to Elsevier Science.
Y1  - 1997///
PB  - Elsevier Science
JF  - Enzyme and Microbial Technology
KW  - Thermoamylase; mutagenesis; cloning; subcloning; plasmid stability
SN  - 01410229
SP  - 525
ER  -