%A G.S. Sidhu
%A P Sharma
%A T Chakrabarti
%A J.K. Gupta
%O Copyright of this article belongs to Elsevier Science.
%J Enzyme and Microbial Technology
%T Strain improvement for the production of a thermostable ?-amylase
%X Bacillus sp. MK716, a thermophilic and amylolytic soil isolate, produced a thermostable ?-amylase active even at 100?C. The enzyme yield was increased 40 times through mutagenesis with ethylmethane sulfonate. The mutated gene was cloned in Escherichia coli-pBR322 system, and the 4.8 kb cloned fragment was mapped with restriction enzymes. Subcloning in Bacillus subtilis of a 2.0 kb portion of the cloned fragment resulted in 107 times increased enzyme yield. The recombinant plasmid was completely stable in B. subtilis.
%N 7
%K Thermoamylase; mutagenesis; cloning; subcloning; plasmid stability
%P 525-530
%V 21
%D 1997
%I Elsevier Science
%R doi:10.1016/S0141-0229(97)00055-0
%L open772