creators_name: Sidhu, G.S.
creators_name: Sharma, P
creators_name: Chakrabarti, T
creators_name: Gupta, J.K.
type: article
datestamp: 2012-01-27 14:57:12
lastmod: 2015-01-19 05:32:34
metadata_visibility: show
title: Strain improvement for the production of a thermostable α-amylase
ispublished: pub
subjects: QR
full_text_status: restricted
keywords: Thermoamylase; mutagenesis; cloning; subcloning; plasmid stability
note: Copyright of this article belongs to Elsevier Science.
abstract: Bacillus sp. MK716, a thermophilic and amylolytic soil isolate, produced a thermostable α-amylase active even at 100°C. The enzyme yield was increased 40 times through mutagenesis with ethylmethane sulfonate. The mutated gene was cloned in Escherichia coli-pBR322 system, and the 4.8 kb cloned fragment was mapped with restriction enzymes. Subcloning in Bacillus subtilis of a 2.0 kb portion of the cloned fragment resulted in 107 times increased enzyme yield. The recombinant plasmid was completely stable in B. subtilis.
date: 1997
date_type: published
publication: Enzyme and Microbial Technology
volume: 21
number: 7
publisher: Elsevier Science
pagerange: 525-530
id_number: doi:10.1016/S0141-0229(97)00055-0
refereed: TRUE
issn: 01410229
official_url: http://dx.doi.org/10.1016/S0141-0229(97)00055-0
related_url_url: http://www.sciencedirect.com/science/article/pii/S0141022997000550
related_url_type: pub
citation:   Sidhu, G.S. and Sharma, P and Chakrabarti, T and Gupta, J.K.  (1997) Strain improvement for the production of a thermostable α-amylase.  Enzyme and Microbial Technology, 21 (7).  pp. 525-530.  ISSN 01410229     
document_url: http://crdd.osdd.net/open/772/1/tapan1997.pdf