%0 Journal Article
%@ 01681656
%A Mohapatra, B.R.
%A Banerjee, U C
%A Bapuji, M
%D 1998
%F open:783
%I Elsevier Science
%J Journal of Biotechnology
%K Activation and deactivation energies; Amylase; Mucor sp.; Spirastrella sp.; Thermostability
%N 1-2
%P 113-117
%T Characterization of a fungal amylase from Mucor sp. associated with the marine sponge Spirastrella sp.
%U http://crdd.osdd.net/open/783/
%V 60
%X A novel amylase was isolated from the Mucor sp. associated with the marine sponge Spirastrella sp., grown at 30°C. The enzyme has an optimum pH of 5.0 and an optimum temperature of 60°C. The half lives of the partially purified enzyme at 55 and 60°C were 120 and 50 min, respectively. The activation and deactivation energies of the partially purified enzyme were 46.60 and 157.05 kJ mol−1, respectively. The enzyme activity was not affected by the addition of 3% NaCl, 10 mM Ca2+ and 25 mM Mg2+, but was strongly inhibited by EDTA.
%Z Copyright of this article belongs to Elsevier Science.