title: Spectral analysis of a nonideal bioreactor for a recombinant fermentation with run-away plasmids
creator: Patnaik, P R
subject: QR Microbiology
description: An earlier application of spectral analysis to an isothermal, perfectly mixed, fed-batch fermentation [11] showed that it provides insight into the performance and reveals significant differences between key extra-cellular and intra-cellular (recombinant) variables. To enable application on a practically useful scale, the methodology has been extended to a bioreactor with temperature variation, incomplete mixing of the broth and Gaussian disturbances. For the same system, #-galactosidase produced by a recombinant E. coli strain, there are again differences between the rDNA and its protein, on the one hand, and the concentration and mass fraction of recombinant cells, on the other. For certain combinations of the dilution rate and the degrees of mixing, the bioreactor is only marginally stable, implying that a sufficiently large disturbance can trigger unstable behavior.  
publisher: Springer Science
date: 1999
type: Article
type: PeerReviewed
format: application/pdf
identifier: http://crdd.osdd.net/open/807/1/patnaik1999.2.pdf
relation: http://dx.doi.org/10.1007/s004490050556
identifier:   Patnaik, P R  (1999) Spectral analysis of a nonideal bioreactor for a recombinant fermentation with run-away plasmids.  Bioprocess Engineering, 20 (1).  p. 31.  ISSN 0178515X     
relation: http://crdd.osdd.net/open/807/