@article{open816,
          volume = {77},
          number = {3},
          author = {P R Patnaik},
            note = {Copyright of this article belongs to Wiley.},
           title = {Plasmid stability analysis in a non-homogeneous bioreactor for a recombinant fed-batch fermentation},
       publisher = {wiley},
            year = {1999},
         journal = {The Canadian Journal of Chemical Engineering},
           pages = {602--607},
        keywords = {fed-batch fermentation;recombinant E. coli;imperfect macromixing;plasmid stability},
             url = {http://crdd.osdd.net/open/816/},
        abstract = {Fed-batch fermentation for tryptophan synthetase by a recombinant Escherichia coli strain has been analyzed through a model incorporating segregational loss of the plasmid pPLc23trpAl and imperfect macromixing of the broth. These features become significant in large fermentation vessels, where fluid circulation in the bioreactor influences the rates of cell growth and productivity. A simple model consisting of two interacting reactors describes the degree of macromixing, which is characterized by the respective (internal) dilution rates. Controlled imperfect macromixing is superior to other methods in arresting, and even reversing, the normal decline in the concentration of recombinant cells, thereby providing a method to exploit the non-homogeneity of the broth in large bioreactors. Some physical implications are discussed.

On a analys{\'e} la fermentation en alimentation discontinue pour la synthetase de tryptophan par une souche de Escherichia coli recombinante au moyen d'un mod{\`e}le incorporant la perte s{\'e}gr{\'e}gationnelle du plasmide pPLc23trppAI et le macrom{\'e}lange imparfait du bouillon. Ces caract{\'e}ristiques deviennent significatives dans les r{\'e}servoirs de fermentation de grande taille, o-la circulation des fluides dans le bior{\'e}acteur influence la vitesse de croissance et la productivit{\'e} des cellules. Un mod{\`e}le simple compos{\'e} de deux r{\'e}acteurs qui interagissent d{\'e}crit le degr{\'e} de macrom{\'e}lange, qui est caract{\'e}ris{\'e} par les vitesses de dilution respectives (internes). Le macrom{\'e}lange imparfait contr{\^o}l{\'e} est sup{\'e}rieur {\`a} celui d'autres m{\'e}thodes pour stopper, et m{\^e}me inverser, le d{\'e}clin normal dans la concentration de cellules recombinantes, ce qui fournit une m{\'e}thode pour exploiter la non-homog{\'e}n{\'e}it{\'e} du bouillon dans les grands bior{\'e}acteurs. Certaines implications physiques sont analys{\'e}es.
}
}