creators_name: Makkar, R S
creators_name: Cameotra, Swaranjit Singh
type: article
datestamp: 2012-01-31 08:42:30
lastmod: 2015-01-08 10:34:12
metadata_visibility: show
title: Structural characterization of a biosurfactant produced by Bacillus subtilis at 45°C
ispublished: pub
subjects: QR
full_text_status: restricted
keywords: Bacillus  - biosurfactant - surfactin - thermophilic 
note: Copyright of this article belongs to Springer Science.
abstract: Structural and biochemical characterization of a biosurfactant produced by Bacillus subtilis under thermophilic conditions was performed. Preliminary structural determination of CHCl3/CH3OH (65∶15) extracts by thin-layer chromatographic reagents showed it to be identical to surfactin. Also, the infrared, 1H nuclear magnetic resonance, and mass spectroscopy analysis confirmed it to be identical to surfactin. Biochemically, the surfactant was a lipopeptide-containing lipid (17.05%) and protein (13.2%). The surfactant yielded a minimal aqueous surface-tension value of 28 dyne/cm and an interfacial tension value at 0.1% concentration of 0.2 dyne/cm against diesel oil. The critical micelle concentration of the surfactant was 35 mg/L. The biosurfactant exhibited an emulsification value (E 24) of 90 against diesel oil and a sand-pack oil recovery of 62%. It has potential application in microbial-enhanced oil recovery in thermophilic, alkaline, acidic, and halophilic environments
date: 1999
date_type: published
publication: Journal of Surfactants and Detergents
volume: 2
number: 3
publisher: Springer Science
pagerange: 367-372
id_number: doi:10.1007/s11743-999-0091-6
refereed: TRUE
issn: 1097-3958
official_url: http://dx.doi.org/10.1007/s11743-999-0091-6
related_url_url: http://www.springerlink.com/content/dwt26n1x5534738h/
related_url_type: pub
citation:   Makkar, R S and Cameotra, Swaranjit Singh  (1999) Structural characterization of a biosurfactant produced by Bacillus subtilis at 45°C.  Journal of Surfactants and Detergents, 2 (3).  pp. 367-372.  ISSN 1097-3958     
document_url: http://crdd.osdd.net/open/821/1/swaranjit1999.1.pdf