<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Two-step purification of d(-)-specific carbamoylase from Agrobacterium tumefaciens AM 10."^^ . "A simple, economical and rapid affinity chromatography procedure with red dye as a ligand has been described for the two-step purification of a relatively thermostable d(-)-carbamoylase from the cell-free extract of Agrobacterium tumefaciens AM 10. The enzyme was purified 232-fold to homogeneity with a recovery of 30% in the presence of 2 mM dithiothreitol. The specific activity of the enzyme was 7.88 U/mg protein. The enzyme is a dimer with a native molecular mass of 67 kDa and a subunit relative molecular mass of 38 kDa. The isoelectric point of the enzyme was found to be 5.83. The K(m) values for N-carbamoyl-dl-methionine and N-carbamoyl-d-phenylglycine were 3.84 and 5.0 mM, respectively."^^ . "2001-02" . . "21" . "1" . . "Elsevier Science"^^ . . . "Protein expression and purification"^^ . . . "10465928" . . . . . . . . . . . . . . . . "H S"^^ . "Nandanwar"^^ . "H S Nandanwar"^^ . . "R"^^ . "Sharma"^^ . "R Sharma"^^ . . "R M"^^ . "Vohra"^^ . "R M Vohra"^^ . . "D"^^ . "Sareen"^^ . "D Sareen"^^ . . . . . . "Two-step purification of d(-)-specific carbamoylase from Agrobacterium tumefaciens AM 10. (PDF)"^^ . . . . . "Two-step purification of d(-)-specific carbamoylase from Agrobacterium tumefaciens AM 10. (Indexer Terms)"^^ . . . . . . "Two-step purification of d(-)-specific carbamoylase from Agrobacterium tumefaciens AM 10. (Image (PNG))"^^ . . . . . . "Two-step purification of d(-)-specific carbamoylase from Agrobacterium tumefaciens AM 10. (Image (PNG))"^^ . . . . . . "Two-step purification of d(-)-specific carbamoylase from Agrobacterium tumefaciens AM 10. (Image (PNG))"^^ . . . . . . "Two-step purification of d(-)-specific carbamoylase from Agrobacterium tumefaciens AM 10. (Image (PNG))"^^ . . . . . "HTML Summary of #840 \n\nTwo-step purification of d(-)-specific carbamoylase from Agrobacterium tumefaciens AM 10.\n\n" . "text/html" . . . "QR Microbiology"@en . .