creators_name: Naresh, Alpana
creators_name: Singh, Jagmohan
type: article
datestamp: 2012-02-02 16:32:25
lastmod: 2012-02-02 16:32:25
metadata_visibility: show
title: Identification of four genes involved in suppression of the pre-mRNA splicing defect in the sng1-1/rhp6- mutant of fission yeast 
ispublished: pub
subjects: QH426
full_text_status: public
abstract: Apart from the global regulators of silencing in the fission yeast Schizosaccharomyces pombe, namely swi6, clr1,
clr2, clr3, clr4 and rik1, the DNA repair gene rhp6 plays a unique role in mating-type silencing. Recently, we showed
that sng1-1, a mutation in the 5' splice junction of the second intron of the rhp6 gene, leads to derepression of both the
silent loci mat2 and mat3 in switching background. To address the mechanism of rhp6 in silencing, we have isolated
several extragenic suppressors of the sng1-1/rhp6– mutation. These suppressors fall into four complementation groups
and are referred to as suppressor of rhp6: sur1, sur2, sur3 and sur4. Interestingly, reverse transcriptase polymerase
chain reaction analysis of the rhp6 transcript shows that in contrast to about > 50% level of unspliced rhp6 pre-mRNA
in the sng1-1/rhp6– mutant, there is a restoration of normal splicing to varying degrees in the suppressors. The sur2 gene belongs to the AAA-ATPase family of proteins, with maximum homology to the SIN1-associated protein SAP1
of Saccharomyces cerevisiae. We propose that sur2, along with sur1, sur3 and sur4, may play an as yet uncharacterized
role in pre-mRNA splicing.
date: 2000
date_type: published
publication: Journal of Genetics
volume: 79
publisher: IAS
pagerange: 83-90
refereed: TRUE
official_url: http://www.ias.ac.in/jgenet/Vol79No3/JG473.PDF
related_url_url: http://www.ias.ac.in/jgenet/Vol79No3/JG473.PDF
related_url_type: pub
citation:   Naresh, Alpana and Singh, Jagmohan  (2000) Identification of four genes involved in suppression of the pre-mRNA splicing defect in the sng1-1/rhp6- mutant of fission yeast.  Journal of Genetics, 79.  pp. 83-90.      
document_url: http://crdd.osdd.net/open/841/1/jagmohan2000.pdf