TY  - JOUR
N1  - Copyright of this article belongs to National Research Council.
ID  - open850
UR  - http://crdd.osdd.net/open/850/
IS  - 1
A1  - Samanta, S K
A1  - Jain, R K
Y1  - 2000/01//
N2  - A naphthalene (Nap) and salicylate (Sal) degrading microorganism, Pseudomonas putida RKJ1, is chemotactic towards these compounds. This strain carries a 83 kb plasmid. A 25 kb EcoRI fragment of the plasmid contains the genes responsible for Nap degradation through Sal. RKJ5, the plasmid-cured derivative of RKJ1, is neither capable of degradation nor is chemotactic towards Nap or Sal. The recombinant plasmid pRKJ3, which contained a 25 kb EcoRI fragment, was transferred back into the plasmid-free wild-type strain RKJ5, and the transconjugant showed both degradation and chemotaxis. The recombinant plasmid pRKJ3 was also transferred into motile, plasmid-free P. putida KT2442. The resulting transconjugant (RKJ15) showed chemotaxis towards both Nap and Sal. Two mutant strains carrying deletions in pRKJ3 (in KT2442) with phenotypes Nap- Sal+ and Nap- Sal-, were also tested for chemotaxis. It was found that the Nap- Sal+ mutant strain showed chemotaxis towards Sal only, whereas the Nap- Sal- mutant strain is non-chemotactic towards both the compounds. These results suggest that the metabolism of Nap and Sal may be required for the chemotactic activity.
PB  - National Research Council
JF  - Canadian journal of microbiology
VL  - 46
SN  - 0008-4166
TI  - Evidence for plasmid-mediated chemotaxis of Pseudomonas putida towards naphthalene and salicylate.
SP  - 1
AV  - none
EP  - 6
ER  -