creators_name: Samanta, S K
creators_name: Jain, R K
type: article
datestamp: 2012-02-02 16:31:10
lastmod: 2012-02-02 16:31:10
metadata_visibility: show
title: Evidence for plasmid-mediated chemotaxis of Pseudomonas putida towards naphthalene and salicylate.
ispublished: pub
subjects: QR
full_text_status: none
note: Copyright of this article belongs to National Research Council.
abstract: A naphthalene (Nap) and salicylate (Sal) degrading microorganism, Pseudomonas putida RKJ1, is chemotactic towards these compounds. This strain carries a 83 kb plasmid. A 25 kb EcoRI fragment of the plasmid contains the genes responsible for Nap degradation through Sal. RKJ5, the plasmid-cured derivative of RKJ1, is neither capable of degradation nor is chemotactic towards Nap or Sal. The recombinant plasmid pRKJ3, which contained a 25 kb EcoRI fragment, was transferred back into the plasmid-free wild-type strain RKJ5, and the transconjugant showed both degradation and chemotaxis. The recombinant plasmid pRKJ3 was also transferred into motile, plasmid-free P. putida KT2442. The resulting transconjugant (RKJ15) showed chemotaxis towards both Nap and Sal. Two mutant strains carrying deletions in pRKJ3 (in KT2442) with phenotypes Nap- Sal+ and Nap- Sal-, were also tested for chemotaxis. It was found that the Nap- Sal+ mutant strain showed chemotaxis towards Sal only, whereas the Nap- Sal- mutant strain is non-chemotactic towards both the compounds. These results suggest that the metabolism of Nap and Sal may be required for the chemotactic activity.
date: 2000-01
date_type: published
publication: Canadian journal of microbiology
volume: 46
number: 1
publisher: National Research Council
pagerange: 1-6
refereed: TRUE
issn: 0008-4166
citation:   Samanta, S K and Jain, R K  (2000) Evidence for plasmid-mediated chemotaxis of Pseudomonas putida towards naphthalene and salicylate.  Canadian journal of microbiology, 46 (1).  pp. 1-6.  ISSN 0008-4166