TY  - JOUR
ID  - open856
UR  - http://dx.doi.org/10.1016/S0304-4017(01)00502-7
IS  - 2
A1  - Singh, S.
A1  - Khatri, Neeraj
A1  - Manuja, A.
A1  - Sharma, R.D.
A1  - Malhotra, D.V.
A1  - Nichani, A.K.
N2  - Tropical theileriosis, caused by Theileria annulata, is an important tick-borne disease of cattle. A cell culture attenuated vaccine has been developed in our laboratory by long-term in vitro propagation of the schizont stage of the parasite. A longitudinal study was conducted at selected farms housing indigenous, cross-bred and exotic animals to investigate the effect of vaccination on the epidemiology of the disease. A total of 120 animals in 4 age groups were vaccinated with the vaccine before the onset of disease season. An equal number of age-matched animals were kept as controls at the same sites. Animals were monitored for 14 months at monthly intervals. The 97.5% vaccinated animals showed a rise in antibody titres 1 month post-vaccination, as determined by single dilution ELISA. The 78.3% of non-vaccinated animals became sero-positive over the period of observation. Mean antibody titres were significantly higher in vaccinated than non-vaccinated animals. Cross-bred animals showed higher antibody titres followed by exotic and indigenous animals in both the vaccinated and non-vaccinated groups. However, the antibody titres in animals of different ages were similar. The 36.7% vaccinated and 64.2% non-vaccinated animals became carriers (<0.5% piroplasms in erythrocytes) during the observation period. Clinical cases of theileriosis were recorded only in the non-vaccinated group suggesting that vaccinated animals were sufficiently immune to withstand field tick challenge for at least 14 months.
VL  - 101
TI  - Impact of field vaccination with a Theileria annulata schizont cell culture vaccine on the epidemiology of tropical theileriosis
AV  - restricted
EP  - 100
N1  - Copyright of this article belongs to Elsevier Science.
Y1  - 2001///
PB  - Elsevier Science
JF  - Veterinary Parasitology
KW  - Theileria annulata; Field vaccination; Epidemiology
SN  - 03044017
SP  - 91
ER  -