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primary topic: Chromodomain Protein Swi6-mediated Role of DNA Polymerase α in Establishment of Silencing in Fission Yeast

Chromodomain Protein Swi6-mediated Role of DNA Polymerase α in Establishment of Silencing in Fission Yeast
type: AcademicArticle
type: Article
abstract: Although DNA replication has been thought to play an important role in the silencing of mating type loci inSaccharomyces cerevisiae, recent studies indicate that silencing can be decoupled from replication. InSchizosaccharomyces pombe, mating type silencing is brought about by the trans-acting proteins, namely Swi6, Clr1-Clr4, and Rhp6, in cooperation with the cis-acting silencers. The latter contain an autonomous replication sequence, suggesting that DNA replication may be critical for silencing in S. pombe. To investigate the connection between DNA replication and silencing inS. pombe, we analyzed several temperature-sensitive mutants of DNA polymerase α. We find that one such mutant,swi7H4, exhibits silencing defects at mat, centromere, and telomere loci. This effect is independent of the checkpoint and replication defects of the mutant. Interestingly, the extent of the silencing defect in the swi7H4 mutant at the silent mat2 locus is further enhanced in absence of the cis-acting, centromere-proximal silencer. The chromodomain protein Swi6, which is required for silencing and is localized tomat and other heterochromatin loci, interacts with DNA polymerase α in vivo and in vitro in wild type cells. However, it does not interact with the mutant polα and is delocalized away from the silent mat loci in the mutant. Our results demonstrate a role of DNA polymerase α in the establishment of silencing. We propose a recruitment model for the coupling of DNA replication with the establishment of silencing by the chromodomain protein Swi6, which may be applicable to higher eukaryotes.
date: 2011-12-21
volume: 276
is part of: Journal of Biological Chemistry
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
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subject: QD Chemistry

Chromodomain Protein Swi6-mediated Role of DNA Polymerase α in Establishment of Silencing in Fission Yeast (PDF)
type: Document
has file: jagmohan2001.pdf
has part: jagmohan2001.pdf

ASBMB
type: Organization

Journal of Biological Chemistry
type: Collection
issn: 00219258

Shakil Ahmed
type: Person
given name: Shakil
family name: Ahmed

Jagmohan Singh
type: Person
given name: Jagmohan
family name: Singh

Sumit Arora
type: Person
given name: Sumit
family name: Arora

Sharanjot Saini
type: Person
given name: Sharanjot
family name: Saini

Chromodomain Protein Swi6-mediated Role of DNA Polymerase α in Establishment of Silencing in Fission Yeast (Indexer Terms)
type: Document
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Chromodomain Protein Swi6-mediated Role of DNA Polymerase α in Establishment of Silencing in Fission Yeast (Image (PNG))
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Chromodomain Protein Swi6-mediated Role of DNA Polymerase α in Establishment of Silencing in Fission Yeast (Image (PNG))
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primary topic: Chromodomain Protein Swi6-mediated Role of DNA Polymerase α in Establishment of Silencing in Fission Yeast

QD Chemistry
type: Concept