title: Enhancement of protein activity in a recombinant fermentation by optimizing fluid dispersion and initial plasmid copy number distribution
creator: Patnaik, P R
subject: QD Chemistry
description: Recombinant fermentations carried out in large bioreactors are characterized by incomplete dispersion of fluid and a distribution of plasmid copy numbers among the cells. The effects of both features have been studied for the batch production of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by E. coli harboring the plasmid pBR Eco gap. GAPDH activity is maximized by a large average copy number (within metabolically permissible limits) of the cell population and intermediate optimal values of its variance and of dispersion in the broth (expressed by the Peclet number). The optimum conditions, however, should be chosen judiciously because, although GAPDH activities are high, the incremental benefits are lower than under non-optimal operation.
publisher: Elsevier Science
date: 2001
type: Article
type: PeerReviewed
format: application/pdf
identifier: http://crdd.osdd.net/open/872/1/patnaik2001.6.pdf
relation: http://dx.doi.org/10.1016/S1369-703X(01)00130-9
identifier:   Patnaik, P R  (2001) Enhancement of protein activity in a recombinant fermentation by optimizing fluid dispersion and initial plasmid copy number distribution.  Biochemical Engineering Journal, 9 (2).  pp. 111-118.  ISSN 1369703X     
relation: http://crdd.osdd.net/open/872/