title: Synthesis of enhanced biosurfactant by Bacillus subtilis MTCC 2423 at 45°C by foam fractionation
creator: Makkar, R S
creator: Cameotra, Swaranjit Singh
subject: QR Microbiology
description: fermentor was carried out using Bacillus subtilis  MTCC 2423 at 45°C. A good yield was obtained from sucrose  (2%) substrate fermentation by continuous removal of the  product by foam fractionation. The biosurfactant was recovered from collapsed foam by acid precipitation. The biosurfactant yield (4.5 g/L) was about 4.5 times higher than the yield (ca. 1 g/L) obtained by shake-flask fermentation. Surface activity of the collapsed foam was very high, and total surface activity was observed in the collapsed foam. The structural characterization  of this biosurfactant produced at 45°C by the strain used  in this study was recently reported. The biosurfactant was analyzed by thin-layer chromatography, infrared, 1H nuclear  magnetic resonance, and mass spectroscopy and was found  to be identical to surfactin, a lipopeptide surfactant.
publisher: Springer Science
date: 2001
type: Article
type: PeerReviewed
format: application/pdf
identifier: http://crdd.osdd.net/open/875/1/swaranjit2001.pdf
relation: http://dx.doi.org/10.1007/s11743-001-0187-z
identifier:   Makkar, R S and Cameotra, Swaranjit Singh  (2001) Synthesis of enhanced biosurfactant by Bacillus subtilis MTCC 2423 at 45°C by foam fractionation.  Journal of Surfactants and Detergents, 4 (4).  pp. 355-357.  ISSN 1097-3958     
relation: http://crdd.osdd.net/open/875/