TY - JOUR ID - open875 UR - http://dx.doi.org/10.1007/s11743-001-0187-z IS - 4 A1 - Makkar, R S A1 - Cameotra, Swaranjit Singh N2 - fermentor was carried out using Bacillus subtilis MTCC 2423 at 45°C. A good yield was obtained from sucrose (2%) substrate fermentation by continuous removal of the product by foam fractionation. The biosurfactant was recovered from collapsed foam by acid precipitation. The biosurfactant yield (4.5 g/L) was about 4.5 times higher than the yield (ca. 1 g/L) obtained by shake-flask fermentation. Surface activity of the collapsed foam was very high, and total surface activity was observed in the collapsed foam. The structural characterization of this biosurfactant produced at 45°C by the strain used in this study was recently reported. The biosurfactant was analyzed by thin-layer chromatography, infrared, 1H nuclear magnetic resonance, and mass spectroscopy and was found to be identical to surfactin, a lipopeptide surfactant. VL - 4 TI - Synthesis of enhanced biosurfactant by Bacillus subtilis MTCC 2423 at 45°C by foam fractionation AV - restricted EP - 357 N1 - Copyright of this article belongs to Springer Science. Y1 - 2001/// PB - Springer Science JF - Journal of Surfactants and Detergents KW - Bacillus KW - enhanced biosurfactant KW - foam fractionation KW - surfactin KW - thermophilic. SN - 1097-3958 SP - 355 ER -