creators_name: Makkar, R S creators_name: Cameotra, Swaranjit Singh type: article datestamp: 2012-02-03 16:13:04 lastmod: 2012-03-29 06:37:18 metadata_visibility: show title: Synthesis of enhanced biosurfactant by Bacillus subtilis MTCC 2423 at 45°C by foam fractionation ispublished: pub subjects: QR full_text_status: restricted keywords: Bacillus, enhanced biosurfactant, foam fractionation, surfactin, thermophilic. note: Copyright of this article belongs to Springer Science. abstract: fermentor was carried out using Bacillus subtilis MTCC 2423 at 45°C. A good yield was obtained from sucrose (2%) substrate fermentation by continuous removal of the product by foam fractionation. The biosurfactant was recovered from collapsed foam by acid precipitation. The biosurfactant yield (4.5 g/L) was about 4.5 times higher than the yield (ca. 1 g/L) obtained by shake-flask fermentation. Surface activity of the collapsed foam was very high, and total surface activity was observed in the collapsed foam. The structural characterization of this biosurfactant produced at 45°C by the strain used in this study was recently reported. The biosurfactant was analyzed by thin-layer chromatography, infrared, 1H nuclear magnetic resonance, and mass spectroscopy and was found to be identical to surfactin, a lipopeptide surfactant. date: 2001 date_type: published publication: Journal of Surfactants and Detergents volume: 4 number: 4 publisher: Springer Science pagerange: 355-357 id_number: doi:10.1007/s11743-001-0187-z refereed: TRUE issn: 1097-3958 official_url: http://dx.doi.org/10.1007/s11743-001-0187-z related_url_url: http://www.springerlink.com/content/446k64l216673772/ related_url_type: pub citation: Makkar, R S and Cameotra, Swaranjit Singh (2001) Synthesis of enhanced biosurfactant by Bacillus subtilis MTCC 2423 at 45°C by foam fractionation. Journal of Surfactants and Detergents, 4 (4). pp. 355-357. ISSN 1097-3958 document_url: http://crdd.osdd.net/open/875/1/swaranjit2001.pdf