<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Matrix-assisted laser desorption ionization--time of flight mass spectrometry of lipopeptide biosurfactants in whole cells and culture filtrates of Bacillus subtilis C-1 isolated from petroleum sludge."^^ . "An innovative method was developed for rapid sensitive detection and efficient structural characterization of lipopeptide biosurfactants by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry by using whole microbial cells and crude culture filtrates as targets in combination with surface tension measurements. This was done for a bacterial strain that was isolated from petroleum sludge and efficiently produces biosurfactants. This organism was identified by using biochemical, physiological, and genetic parameters as a Bacillus subtilis strain, designated B. subtilis C-1. This assignment was supported by a mass spectrometric investigation of the secondary metabolite spectrum determined by whole-cell MALDI-TOF mass spectrometry, which revealed three lipopeptide complexes, the surfactins, the iturins, and the fengycins, which are well-known biosurfactants produced by B. subtilis strains. These compounds were structurally characterized by in situ structure analysis by using postsource decay MALDI-TOF mass spectrometry. The isoforms were separated by miniaturized high-resolution reversed-phase high-performance liquid chromatography for mass spectrometric characterization. Iturin compounds which contain unusual fatty acid components were detected."^^ . "2002-12" . . "68" . "12" . . "ASM"^^ . . . "Applied and environmental microbiology"^^ . . . "00992240" . . . . . . . . . . . . . . . . . . . . . . "Neena"^^ . "Mehta"^^ . "Neena Mehta"^^ . . "Christopher"^^ . "Wilde"^^ . "Christopher Wilde"^^ . . "Swaranjit Singh"^^ . "Cameotra"^^ . "Swaranjit Singh Cameotra"^^ . . "Bärbel"^^ . "Kablitz"^^ . "Bärbel Kablitz"^^ . . "Peter"^^ . "Franke"^^ . "Peter Franke"^^ . . "Joachim"^^ . "Vater"^^ . "Joachim Vater"^^ . . . . . . "Matrix-assisted laser desorption ionization--time of flight mass spectrometry of lipopeptide biosurfactants in whole cells and culture filtrates of Bacillus subtilis C-1 isolated from petroleum sludge. (PDF)"^^ . . . . . "Matrix-assisted laser desorption ionization--time of flight mass spectrometry of lipopeptide biosurfactants in whole cells and culture filtrates of Bacillus subtilis C-1 isolated from petroleum sludge. (Image (PNG))"^^ . . . . . . "Matrix-assisted laser desorption ionization--time of flight mass spectrometry of lipopeptide biosurfactants in whole cells and culture filtrates of Bacillus subtilis C-1 isolated from petroleum sludge. (Image (PNG))"^^ . . . . . . "Matrix-assisted laser desorption ionization--time of flight mass spectrometry of lipopeptide biosurfactants in whole cells and culture filtrates of Bacillus subtilis C-1 isolated from petroleum sludge. (Image (PNG))"^^ . . . . . . "Matrix-assisted laser desorption ionization--time of flight mass spectrometry of lipopeptide biosurfactants in whole cells and culture filtrates of Bacillus subtilis C-1 isolated from petroleum sludge. (Image (PNG))"^^ . . . . . . "Matrix-assisted laser desorption ionization--time of flight mass spectrometry of lipopeptide biosurfactants in whole cells and culture filtrates of Bacillus subtilis C-1 isolated from petroleum sludge. (Indexer Terms)"^^ . . . . . "HTML Summary of #894 \n\nMatrix-assisted laser desorption ionization--time of flight mass spectrometry of lipopeptide biosurfactants in whole cells and culture filtrates of Bacillus subtilis C-1 isolated from petroleum sludge.\n\n" . "text/html" . . . "QR Microbiology"@en . .