TY  - JOUR
N1  - Copyright of this article belongs to ASM.
ID  - open894
UR  - http://aem.asm.org/content/68/12/6210.full.pdf+html
IS  - 12
A1  - Vater, Joachim
A1  - Kablitz, Bärbel
A1  - Wilde, Christopher
A1  - Franke, Peter
A1  - Mehta, Neena
A1  - Cameotra, Swaranjit Singh
Y1  - 2002/12//
N2  - An innovative method was developed for rapid sensitive detection and efficient structural characterization of lipopeptide biosurfactants by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry by using whole microbial cells and crude culture filtrates as targets in combination with surface tension measurements. This was done for a bacterial strain that was isolated from petroleum sludge and efficiently produces biosurfactants. This organism was identified by using biochemical, physiological, and genetic parameters as a Bacillus subtilis strain, designated B. subtilis C-1. This assignment was supported by a mass spectrometric investigation of the secondary metabolite spectrum determined by whole-cell MALDI-TOF mass spectrometry, which revealed three lipopeptide complexes, the surfactins, the iturins, and the fengycins, which are well-known biosurfactants produced by B. subtilis strains. These compounds were structurally characterized by in situ structure analysis by using postsource decay MALDI-TOF mass spectrometry. The isoforms were separated by miniaturized high-resolution reversed-phase high-performance liquid chromatography for mass spectrometric characterization. Iturin compounds which contain unusual fatty acid components were detected.
PB  - ASM
JF  - Applied and environmental microbiology
VL  - 68
SN  - 0099-2240
TI  - Matrix-assisted laser desorption ionization--time of flight mass spectrometry of lipopeptide biosurfactants in whole cells and culture filtrates of Bacillus subtilis C-1 isolated from petroleum sludge.
SP  - 6210
AV  - restricted
EP  - 9
ER  -