<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme"^^ . "Overproduction of cellulolytic enzymes through conventional nuclear transformation approaches posed a major\r\nchallenge as they can potentially degrade the cell wall components and thereby affect transgenic plant growth\r\nand development. In this study, we have tested the possibility to over produce an alkali-thermostable xylanase\r\ngene from Bacillus sp. Strain NG-27 in tobacco plants through chloroplast expression. Our results showed that\r\nthe xylanase expression can reach up to 6% of the total soluble protein, a value comparable to high level expression\r\nreported for several non-cellulolytic proteins in tobacco chloroplasts. The chloroplast-expressed xylanase\r\nretained its activity even when the leaves were dried under sun or at 42 °C, offering flexibility in the\r\nagricultural system in transport and storage. The recombinant enzyme was purified to homogeneity using single\r\nstep chromatography with more than 85% recovery. Most importantly, transgenic plants were indistinguishable\r\nfrom the control untransformed plants in their morphology, growth and in seed setting. These results open up\r\nnew avenues for large scale production of several other industrially useful cellulolytic enzymes through chloroplast\r\nexpression."^^ . "2003" . . . "11" . "1" . . "Springer Science"^^ . . . "Molecular Breeding"^^ . . . "13803743" . . . . . . . . . . . . . . . . . . . "Vanga"^^ . "Siva Reddy"^^ . "Vanga Siva Reddy"^^ . . "Sadhu"^^ . "Leelavathi"^^ . "Sadhu Leelavathi"^^ . . "Shankar"^^ . "Maiti"^^ . "Shankar Maiti"^^ . . "Naveen"^^ . "Gupta"^^ . "Naveen Gupta"^^ . . "Amit"^^ . "Ghosh"^^ . "Amit Ghosh"^^ . . . . . . "Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (PDF)"^^ . . . . . "Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (Indexer Terms)"^^ . . . . . . "Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (Image (PNG))"^^ . . . . . . "Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (Image (PNG))"^^ . . . . . . "Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (Image (PNG))"^^ . . . . . . "Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (Image (PNG))"^^ . . . . . "HTML Summary of #897 \n\nOverproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme\n\n" . "text/html" . . . "QR Microbiology"@en . .