…
comment: The repository administrator has not yet configured an RDF license.
primary topic: Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme

Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme
type: AcademicArticle
type: Article
abstract: Overproduction of cellulolytic enzymes through conventional nuclear transformation approaches posed a major challenge as they can potentially degrade the cell wall components and thereby affect transgenic plant growth and development. In this study, we have tested the possibility to over produce an alkali-thermostable xylanase gene from Bacillus sp. Strain NG-27 in tobacco plants through chloroplast expression. Our results showed that the xylanase expression can reach up to 6% of the total soluble protein, a value comparable to high level expression reported for several non-cellulolytic proteins in tobacco chloroplasts. The chloroplast-expressed xylanase retained its activity even when the leaves were dried under sun or at 42 °C, offering flexibility in the agricultural system in transport and storage. The recombinant enzyme was purified to homogeneity using single step chromatography with more than 85% recovery. Most importantly, transgenic plants were indistinguishable from the control untransformed plants in their morphology, growth and in seed setting. These results open up new avenues for large scale production of several other industrially useful cellulolytic enzymes through chloroplast expression.
date: 2003
volume: 11
issue: 1
is part of: Molecular Breeding
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
has document: Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (PDF)
has version: Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (PDF)
has published: Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (PDF)
has document: Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (Indexer Terms)
has document: Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (Image (PNG))
subject: QR Microbiology

Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (PDF)
type: Document

Springer Science
type: Organization

Molecular Breeding
type: Collection
issn: 13803743

Vanga Siva Reddy
type: Person
given name: Vanga
family name: Siva Reddy

Sadhu Leelavathi
type: Person
given name: Sadhu
family name: Leelavathi

Shankar Maiti
type: Person
given name: Shankar
family name: Maiti

Naveen Gupta
type: Person
given name: Naveen
family name: Gupta

Amit Ghosh
type: Person
given name: Amit
family name: Ghosh

Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (Indexer Terms)
type: Document
is version of: Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (PDF)
is volatile version of: Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (PDF)
is index codes version of: Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (PDF)

Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (Image (PNG))
type: Document
is version of: Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (PDF)
is volatile version of: Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (PDF)
islightbox thumbnail version of: Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (PDF)

Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (Image (PNG))
type: Document
is version of: Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (PDF)
is volatile version of: Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (PDF)
ispreview thumbnail version of: Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (PDF)

Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (Image (PNG))
type: Document
is version of: Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (PDF)
is volatile version of: Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (PDF)
ismedium thumbnail version of: Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (PDF)

Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (Image (PNG))
type: Document
is version of: Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (PDF)
is volatile version of: Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (PDF)
issmall thumbnail version of: Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme (PDF)

HTML Summary of #897 Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme
format: text/html
primary topic: Overproduction of an alkali- and thermo-stable xylanase in tobacco chloroplasts and efficient recovery of the enzyme

QR Microbiology
type: Concept