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primary topic: Sandwich microgravimetric immunoassay: Sensitive and specific detection of low molecular weight analytes using piezoelectric quartz crystal

Sandwich microgravimetric immunoassay: Sensitive and specific detection of low molecular weight analytes using piezoelectric quartz crystal
type: AcademicArticle
type: Article
abstract: A multi-channel sandwich microgravimetric immunoassay (sMIA), using the quartz crystal microbalance (QCM) principle, has been developed to quantify low molecular weight substances in standard solutions. An antigen is sandwiched between two antigen-specific antibodies: the first antibody is coated on the quartz crystal surface and the second antibody is used for the detection of analyte. The concentration of low molecular weight antigen (insulin was used in this study, Mr ∼ 6000 Da) was correlated with the shift of resonant frequency of QCM system before and after second antibody binding to insulin. The developed assay is highly specific showing low cross-reactivity, and is sensitive to approx. 1 ng insulin ml−1 with a linear response for insulin from 10 μg ml−1 to 10 ng ml−1 in standard solutions. The technique may also be applied for the detection of other small biomolecules
date: 2002
volume: 24
issue: 9
is part of: Biotechnology Letters
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
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subject: QR Microbiology

Sandwich microgravimetric immunoassay: Sensitive and specific detection of low molecular weight analytes using piezoelectric quartz crystal (PDF)
type: Document

Springer Science
type: Organization

Biotechnology Letters
type: Collection
issn: 01415492

Satish Saha
type: Person
given name: Satish
family name: Saha

Manoj Raje
type: Person
given name: Manoj
family name: Raje

C Raman Suri
type: Person
given name: C Raman
family name: Suri

Sandwich microgravimetric immunoassay: Sensitive and specific detection of low molecular weight analytes using piezoelectric quartz crystal (Indexer Terms)
type: Document
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primary topic: Sandwich microgravimetric immunoassay: Sensitive and specific detection of low molecular weight analytes using piezoelectric quartz crystal

QR Microbiology
type: Concept