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Sandwich microgravimetric immunoassay: Sensitive and specific detection of low molecular weight analytes using piezoelectric quartz crystal
A multi-channel sandwich microgravimetric immunoassay (sMIA), using the quartz crystal microbalance (QCM) principle, has been developed to quantify low molecular weight substances in standard solutions. An antigen is sandwiched between two antigen-specific antibodies: the first antibody is coated on the quartz crystal surface and the second antibody is used for the detection of analyte. The concentration of low molecular weight antigen (insulin was used in this study, Mr ∼ 6000 Da) was correlated with the shift of resonant frequency of QCM system before and after second antibody binding to insulin. The developed assay is highly specific showing low cross-reactivity, and is sensitive to approx. 1 ng insulin ml−1 with a linear response for insulin from 10 μg ml−1 to 10 ng ml−1 in standard solutions. The technique may also be applied for the detection of other small biomolecules
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QR Microbiology