TY  - JOUR
ID  - open902
UR  - http://dx.doi.org/10.1023/A:1015238201367
IS  - 9
A1  - Saha, Satish
A1  - Raje, Manoj
A1  - Suri, C Raman
N2  - A multi-channel sandwich microgravimetric immunoassay (sMIA), using the quartz crystal microbalance (QCM)
principle, has been developed to quantify low molecular weight substances in standard solutions. An antigen is
sandwiched between two antigen-specific antibodies: the first antibody is coated on the quartz crystal surface and
the second antibody is used for the detection of analyte. The concentration of low molecular weight antigen (insulin
was used in this study, Mr ? 6000 Da) was correlated with the shift of resonant frequency of QCM system before
and after second antibody binding to insulin. The developed assay is highly specific showing low cross-reactivity,
and is sensitive to approx. 1 ng insulin ml?1 with a linear response for insulin from 10 ?g ml?1 to 10 ng ml?1 in
standard solutions. The technique may also be applied for the detection of other small biomolecules
VL  - 24
TI  - Sandwich microgravimetric immunoassay: Sensitive and specific detection of low molecular weight analytes using piezoelectric quartz crystal
AV  - restricted
EP  - 716
N1  - Copyright of this article belongs to Springer Science.
Y1  - 2002///
PB  - Springer Science
JF  - Biotechnology Letters
KW  - insulin
KW  -  multi-channel
KW  -  quartz crystal microbalance
SN  - 01415492
SP  - 711
ER  -