%A Satish Saha
%A Manoj Raje
%A C Raman Suri
%O Copyright of this article belongs to Springer Science.
%J Biotechnology Letters
%T Sandwich microgravimetric immunoassay: Sensitive and specific detection of low molecular weight analytes using piezoelectric quartz crystal
%X A multi-channel sandwich microgravimetric immunoassay (sMIA), using the quartz crystal microbalance (QCM)
principle, has been developed to quantify low molecular weight substances in standard solutions. An antigen is
sandwiched between two antigen-specific antibodies: the first antibody is coated on the quartz crystal surface and
the second antibody is used for the detection of analyte. The concentration of low molecular weight antigen (insulin
was used in this study, Mr ? 6000 Da) was correlated with the shift of resonant frequency of QCM system before
and after second antibody binding to insulin. The developed assay is highly specific showing low cross-reactivity,
and is sensitive to approx. 1 ng insulin ml?1 with a linear response for insulin from 10 ?g ml?1 to 10 ng ml?1 in
standard solutions. The technique may also be applied for the detection of other small biomolecules
%N 9
%K insulin, multi-channel, quartz crystal microbalance
%P 711-716
%V 24
%D 2002
%I Springer Science
%R doi:10.1023/A:1015238201367
%L open902