creators_name: Saha, Satish
creators_name: Raje, Manoj
creators_name: Suri, C Raman
type: article
datestamp: 2012-02-06 18:16:58
lastmod: 2015-01-07 04:48:02
metadata_visibility: show
title: Sandwich microgravimetric immunoassay: Sensitive and specific detection of low molecular weight analytes using piezoelectric quartz crystal
ispublished: pub
subjects: QR
full_text_status: restricted
keywords: insulin, multi-channel, quartz crystal microbalance
note: Copyright of this article belongs to Springer Science.
abstract: A multi-channel sandwich microgravimetric immunoassay (sMIA), using the quartz crystal microbalance (QCM)
principle, has been developed to quantify low molecular weight substances in standard solutions. An antigen is
sandwiched between two antigen-specific antibodies: the first antibody is coated on the quartz crystal surface and
the second antibody is used for the detection of analyte. The concentration of low molecular weight antigen (insulin
was used in this study, Mr ∼ 6000 Da) was correlated with the shift of resonant frequency of QCM system before
and after second antibody binding to insulin. The developed assay is highly specific showing low cross-reactivity,
and is sensitive to approx. 1 ng insulin ml−1 with a linear response for insulin from 10 μg ml−1 to 10 ng ml−1 in
standard solutions. The technique may also be applied for the detection of other small biomolecules
date: 2002
date_type: published
publication: Biotechnology Letters
volume: 24
number: 9
publisher: Springer Science
pagerange: 711-716
id_number: doi:10.1023/A:1015238201367
refereed: TRUE
issn: 01415492
official_url: http://dx.doi.org/10.1023/A:1015238201367
related_url_url: http://www.springerlink.com/content/tl0t5yyh9dlkpt75/
related_url_type: pub
citation:   Saha, Satish and Raje, Manoj and Suri, C Raman  (2002) Sandwich microgravimetric immunoassay: Sensitive and specific detection of low molecular weight analytes using piezoelectric quartz crystal.  Biotechnology Letters, 24 (9).  pp. 711-716.  ISSN 01415492     
document_url: http://crdd.osdd.net/open/902/1/suri2002.pdf