<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Production and characterization of a thermostable beta-galactosidase from Bacillus coagulans RCS3."^^ . "A strain of Bacillus coagulans RCS3 isolated from a hot-water spring produced significant beta-galactosidase activity at 10 days of growth in a flask. While enzyme production was maximum at 50 degrees C, the highest activity was at 65 degrees C, where the half-life was 2 h. A 2 degrees C decrease in temperature increased the half-life to 15 h without significantly changing the activity, suggesting that 63 degrees C is the temperature of preference compared with 65 degrees C for a combination of good activity and stability. The beta-galactosidase was also stable over pH 5-8, with peak activity at pH 6-7. It was strongly and competitively inhibited by the hydrolysis product galactose. Bivalent cations (Cu(2+), Ni(2+) and Hg(2+)) in the concentration range of 0.5-2.0 mM also inhibited enzyme activity. Both lactose solution and whey could be hydrolysed substantially within 36 h at 50 degrees C. The thermostability and pH-stability and good hydrolytic capability make this enzyme potentially useful in the dairy industry. "^^ . "2002" . "36" . "Pt 1" . . "Portland Press"^^ . . . "Biotechnology and applied biochemistry"^^ . . . "08854513" . . . . . . . . . . . . . . . . . . . "P R"^^ . "Patnaik"^^ . "P R Patnaik"^^ . . "Ranbir C"^^ . "Sobti"^^ . "Ranbir C Sobti"^^ . . "Navneet"^^ . "Batra"^^ . "Navneet Batra"^^ . . "Jagtar"^^ . "Singh"^^ . "Jagtar Singh"^^ . . "U C"^^ . "Banerjee"^^ . "U C Banerjee"^^ . . . . . "HTML Summary of #909 \n\nProduction and characterization of a thermostable beta-galactosidase from Bacillus coagulans RCS3.\n\n" . "text/html" . . . "QR Microbiology"@en . .