title: Molecular characterization of rice hsp101: complementation of yeast hsp104 mutation by disaggregation of protein granules and differential expression in indica and japonica rice types.
creator: Agarwal, Manu
creator: Sahi, Chandan
creator: Katiyar-Agarwal, Surekha
creator: Agarwal, Sangeeta
creator: Young, Todd
creator: Gallie, Daniel R
creator: Sharma, Vishva Mitra
creator: Ganesan, K
creator: Grover, Anil
subject: QR Microbiology
description: HSP100 protein is an important component of the heat-shock response in diverse organisms. Using specific primers based on cDNA sequence, rice hsp101 gene was PCR-amplified and sequenced. Southern analysis revealed that there appears to be a single gene per haploid genome coding for HSP101 protein in rice. Northern analysis showed that expression of hsp101 transcript is strictly heat-inducible and induction is transient in nature. In the temperature regime tested, 45 degrees C treatment to intact rice seedlings for 2 h showed maximal levels of hsp101 mRNA. Rice full-length hsp101 cDNA complemented yeast mutant disrupted for its own hsp104 gene by insertional mutagenesis, with efficacy that was comparable with Arabidopsis hsp101 cDNA. Electron micrographic evidence suggested that rice hsp101 cDNA in yeast is active in re-solubilizing the stress-induced protein granules in the post-stress recovery period. Rice hsp101 cDNA expression in hsp104 deficient yeast also caused recovery in tolerance against arsenite. Western analyses showed that this protein is expressed more rapidly during the stress period and retained for longer duration in the post-stress recovery period in japonica rice as compared to indica rice types. This is the first report wherein plant HSP100 protein expression is correlated to disappearance of protein granules in the yeast cells and distinct rice type-dependent protein expression patterns are reported.
publisher: Kluwer Academic Publishers/Springer
date: 2003-03
type: Article
type: PeerReviewed
format: application/pdf
identifier: http://crdd.osdd.net/open/934/1/ganesan2003.pdf
relation: http://www.springerlink.com/content/x46137m7p6013267/fulltext.pdf
identifier:   Agarwal, Manu and Sahi, Chandan and Katiyar-Agarwal, Surekha and Agarwal, Sangeeta and Young, Todd and Gallie, Daniel R and Sharma, Vishva Mitra and Ganesan, K and Grover, Anil  (2003) Molecular characterization of rice hsp101: complementation of yeast hsp104 mutation by disaggregation of protein granules and differential expression in indica and japonica rice types.  Plant molecular biology, 51 (4).  pp. 543-53.  ISSN 0167-4412     
relation: http://crdd.osdd.net/open/934/