creators_name: Zhao, Chenhua
creators_name: Yao, Juming
creators_name: Masuda, Hiromi
creators_name: Kishore, Raghuvansh
creators_name: Asakura, Tetsuo
type: article
datestamp: 2012-02-14 10:31:52
lastmod: 2012-02-14 10:31:52
metadata_visibility: show
title: Structural characterization and artificial fiber formation of Bombyx mori silk fibroin in hexafluoro-iso-propanol solvent system.
ispublished: pub
subjects: QD
full_text_status: restricted
keywords: Bombyx mori silk fibroin; hexafluoro-iso-propanol; 13C CP/MAS NMR; 310-helix; 13C chemical shift
note: Copyright of this article belongs to Wiley.
abstract: High-resolution solution (13)C-NMR and CD studies of Bombyx mori silk fibroin revealed the presence of an ordered secondary structure 3(10)-helix, in hexafluoro-iso-propanol (HFIP). The solid-state structure of the silk fibroin film prepared by drying it gently from the HFIP solution still keep the structure, 3(10)-helix, which was studied with high-resolution solid state (13)C-NMR. The structural transition from the 3(10)-helix to silk II structure, heterogeneous structure including antiparallel beta-sheet, occurred during the artificial spinning from the HFIP solution. The wide-angle x-ray diffraction and differential scanning calorimetry thermograms of the artificial spinning fiber after postspinning treatments were observed together with the stress-strain curves. The results emphasize that the molecular structures, controlled morphology, and mechanical properties of the protein-based synthetic polymers can be modulated for enhancing biocompatibility.
date: 2003-06
date_type: published
publication: Biopolymers
volume: 69
number: 2
publisher: wiley
pagerange: 253-9
refereed: TRUE
issn: 0006-3525
official_url: http://onlinelibrary.wiley.com/doi/10.1002/bip.10350/pdf
related_url_url: http://onlinelibrary.wiley.com/doi/10.1002/bip.10350/pdf
related_url_type: pub
citation:   Zhao, Chenhua and Yao, Juming and Masuda, Hiromi and Kishore, Raghuvansh and Asakura, Tetsuo  (2003) Structural characterization and artificial fiber formation of Bombyx mori silk fibroin in hexafluoro-iso-propanol solvent system.  Biopolymers, 69 (2).  pp. 253-9.  ISSN 0006-3525     
document_url: http://crdd.osdd.net/open/937/1/kishore2003.1.pdf