@article{open945,
          volume = {6},
          number = {1},
          author = {V. Pruthi and Swaranjit Singh Cameotra},
            note = {Copyright of this article belongs to Springer Science.},
           title = {Effect of nutrients on optimal production of biosurfactants by Pseudomonas putida{--}A gujarat oil field isolate},
       publisher = {Springer Science},
            year = {2003},
         journal = {Journal of Surfactants and Detergents},
           pages = {65--68},
        keywords = {Biosurfactant, maximum, nutrients, Pseudomonas
putida (a Gujarat oil field isolate).},
             url = {http://crdd.osdd.net/open/945/},
        abstract = {Nutritional requirements for maximal production
of biosurfactant by an oil field bacterium Pseudomonas
putida were determined. The optimal concentrations of nitrogen,phosphate, sulfur, magnesium, iron, potassium, sodium,calcium, and trace elements for maximal production of biosurfactants were ascertained, and a new ?Pruthi and Cameotra? salt medium was formulated. Data show that maximal biomass (2.4 g L?1) and biosurfactant production (6.28 g L?1) takes place after 72 h of growth on 2\% hexadecane. The biosurfactant was produced optimally over pH and temperature ranges of 6.4?7.2 and 30?40?C, respectively. That the highest biosurfactant yield was obtained during late log phase of growth indicates that the biosurfactant is a secondary metabolite. 
}
}