title: Effect of nutrients on optimal production of biosurfactants by Pseudomonas putida—A gujarat oil field isolate
creator: Pruthi, V.
creator: Cameotra, Swaranjit Singh
subject: QR Microbiology
description: Nutritional requirements for maximal production  of biosurfactant by an oil field bacterium Pseudomonas  putida were determined. The optimal concentrations of nitrogen,phosphate, sulfur, magnesium, iron, potassium, sodium,calcium, and trace elements for maximal production of biosurfactants were ascertained, and a new “Pruthi and Cameotra” salt medium was formulated. Data show that maximal biomass (2.4 g L−1) and biosurfactant production (6.28 g L−1) takes place after 72 h of growth on 2% hexadecane. The biosurfactant was produced optimally over pH and temperature ranges of 6.4–7.2 and 30–40°C, respectively. That the highest biosurfactant yield was obtained during late log phase of growth indicates that the biosurfactant is a secondary metabolite.   
publisher: Springer Science
date: 2003
type: Article
type: PeerReviewed
format: application/pdf
identifier: http://crdd.osdd.net/open/945/1/swaranjit2003.pdf
relation: http://dx.doi.org/10.1007/s11743-003-0250-9
identifier:   Pruthi, V. and Cameotra, Swaranjit Singh  (2003) Effect of nutrients on optimal production of biosurfactants by Pseudomonas putida—A gujarat oil field isolate.  Journal of Surfactants and Detergents, 6 (1).  pp. 65-68.  ISSN 1097-3958     
relation: http://crdd.osdd.net/open/945/