creators_name: Pruthi, V.
creators_name: Cameotra, Swaranjit Singh
type: article
datestamp: 2012-02-14 10:31:00
lastmod: 2012-03-29 06:31:43
metadata_visibility: show
title: Effect of nutrients on optimal production of biosurfactants by Pseudomonas putida—A gujarat oil field isolate
ispublished: pub
subjects: QR
full_text_status: restricted
keywords: Biosurfactant, maximum, nutrients, Pseudomonas
putida (a Gujarat oil field isolate).
note: Copyright of this article belongs to Springer Science.
abstract: Nutritional requirements for maximal production
of biosurfactant by an oil field bacterium Pseudomonas
putida were determined. The optimal concentrations of nitrogen,phosphate, sulfur, magnesium, iron, potassium, sodium,calcium, and trace elements for maximal production of biosurfactants were ascertained, and a new “Pruthi and Cameotra” salt medium was formulated. Data show that maximal biomass (2.4 g L−1) and biosurfactant production (6.28 g L−1) takes place after 72 h of growth on 2% hexadecane. The biosurfactant was produced optimally over pH and temperature ranges of 6.4–7.2 and 30–40°C, respectively. That the highest biosurfactant yield was obtained during late log phase of growth indicates that the biosurfactant is a secondary metabolite. 

date: 2003
date_type: published
publication: Journal of Surfactants and Detergents
volume: 6
number: 1
publisher: Springer Science
pagerange: 65-68
id_number: doi:10.1007/s11743-003-0250-9
refereed: TRUE
issn: 1097-3958
official_url: http://dx.doi.org/10.1007/s11743-003-0250-9
related_url_url: http://www.springerlink.com/content/687g86201505t4l0/fulltext.pdf
related_url_type: pub
citation:   Pruthi, V. and Cameotra, Swaranjit Singh  (2003) Effect of nutrients on optimal production of biosurfactants by Pseudomonas putida—A gujarat oil field isolate.  Journal of Surfactants and Detergents, 6 (1).  pp. 65-68.  ISSN 1097-3958     
document_url: http://crdd.osdd.net/open/945/1/swaranjit2003.pdf