TY  - JOUR
ID  - open958
UR  - http://dx.doi.org/10.1016/S0032-9592(03)00217-6
IS  - 9
A1  - Singh, Jasvir
A1  - Vohra, R M
A1  - Sahoo, Debendra K
N2  - Bacillus sphaericus, an obligate alkalophile, overproduces extracellular alkaline proteases. Kinetics of growth and protease production by this organism were analyzed to identify parameters that control protease synthesis. Results showed protease production to be both growth and non-growth associated although a major portion of the enzyme was secreted in post exponential phases. The initial concentration of glucose and nitrogen sources significantly affected cell growth and enzyme secretion. Fed-batch cultures were performed to maximize protease activity in a bioreactor. By using suitable feeding strategies, the protease activity and its productivity in a fed-batch process was increased by 44% over that in batch process, primarily due to the longer maintenance of increased rates of growth and enzyme production by providing continuous and controlled supply of additional substrate and nutrients.
VL  - 39
TI  - Enhanced production of alkaline proteases by Bacillus sphaericus using fed-batch culture
AV  - restricted
EP  - 1101
N1  - Copyright of this article belongs to Elsevier Science.
Y1  - 2004///
PB  - Elsevier Science
JF  - Process Biochemistry
KW  - Bacillus sphaericus; Alkaline protease; Kinetic analysis; Fed-batch culture
SN  - 13595113
SP  - 1093
ER  -