creators_name: Singh, Jasvir
creators_name: Vohra, R M
creators_name: Sahoo, Debendra K
type: article
datestamp: 2012-02-14 10:29:28
lastmod: 2015-01-09 10:50:19
metadata_visibility: show
title: Enhanced production of alkaline proteases by Bacillus sphaericus using fed-batch culture
ispublished: pub
subjects: QD
full_text_status: restricted
keywords: Bacillus sphaericus; Alkaline protease; Kinetic analysis; Fed-batch culture
note: Copyright of this article belongs to Elsevier Science.
abstract: Bacillus sphaericus, an obligate alkalophile, overproduces extracellular alkaline proteases. Kinetics of growth and protease production by this organism were analyzed to identify parameters that control protease synthesis. Results showed protease production to be both growth and non-growth associated although a major portion of the enzyme was secreted in post exponential phases. The initial concentration of glucose and nitrogen sources significantly affected cell growth and enzyme secretion. Fed-batch cultures were performed to maximize protease activity in a bioreactor. By using suitable feeding strategies, the protease activity and its productivity in a fed-batch process was increased by 44% over that in batch process, primarily due to the longer maintenance of increased rates of growth and enzyme production by providing continuous and controlled supply of additional substrate and nutrients.
date: 2004
date_type: published
publication: Process Biochemistry
volume: 39
number: 9
publisher: Elsevier Science
pagerange: 1093-1101
id_number: doi:10.1016/S0032-9592(03)00217-6
refereed: TRUE
issn: 13595113
official_url: http://dx.doi.org/10.1016/S0032-9592(03)00217-6
related_url_url: http://www.sciencedirect.com/science/article/pii/S0032959203002176
related_url_type: pub
citation:   Singh, Jasvir and Vohra, R M and Sahoo, Debendra K  (2004) Enhanced production of alkaline proteases by Bacillus sphaericus using fed-batch culture.  Process Biochemistry, 39 (9).  pp. 1093-1101.  ISSN 13595113     
document_url: http://crdd.osdd.net/open/958/1/sahoo2004.pdf