@article{open966,
          volume = {28},
           title = {Unusual emergence of guate98-like molecular subtype of DEN-3 during 2003 dengue outbreak in Delhi},
          author = {Manoj Kumar and S.T Pasha and V. Mittal and S.C. Arya and N. Agrawal and D. Bhattacharya and Lal Shiv and Arvind Rai},
       publisher = {WHO},
            year = {2004},
           pages = {457--461},
         journal = {Dengue Bulletin},
             url = {http://crdd.osdd.net/open/966/},
        abstract = {With a view to identifying the molecular subtype of the circulating dengue virus responsible for a major
outbreak of dengue fever (DF) / dengue haemorrhagic fever (DHF) in and around Delhi during the postmonsoon
period in 2003, 32 serum samples were collected from clinically suspected cases. These were
subjected to reverse transcription/polymerase chain reaction (RT/PCR) for amplification of 511 bp CPreM
gene region of the dengue virus. Seven specimens, yielding a satisfactory quantum of viral RNA,
were subsequently processed for automated nucleotide sequencing. Five of the seven analysed isolates
showed close DNA sequence homology with Guate96-98 strains of DEN-3 virus, whereas two turned out
to be genotype IV of DEN-2. Earlier, DEN-2 (genotype IV) had been identified as the etiological agent
during a major DF/DHF outbreak in Delhi in 1996 and also in 2000. Though DEN-2 continues to prevail,
DEN-3, having a close sequence homology with Guate96-98 strains, seems to have entered India for the
first time in late 2003, resulting in a major DF/DHF outbreak. How the Guate96-98 strain of DEN-3
entered India remains to be linked epidemiologically.}
}