TY  - JOUR
ID  - open988
UR  - http://dx.doi.org/10.1002/jctb.1194
IS  - 4
A1  - Ganduri, V.S.R.K.
A1  - Ghosh, Sanjoy
A1  - Patnaik, P R
N2  - Fermentations with genetically altered bacteria tend to lose plasmids as the fermentation
progresses. Methods such as two-stage cultivation, cell recycle and the addition of antibiotics are
commonly used to enhance plasmid stability. Here we examine a different method, the regulation of
mixing in the bioreactor. In particular, large bioreactors are considered where uniform mixing is difficult
to achieve and the probability of plasmid loss varies with the specific growth rate. For both batch and
continuous cultivations of Escherichia coli C600 gal K containing the plasmid pBR Eco gap, it is seen
through a model that bothmodes of operation exhibit high plasmid stability and cell growth when the broth
is incompletely mixed, and mixing near and away from the point of inoculation are unequal. Thus, the
natural incomplete mixing in large bioreactors may be utilized to improve plasmid stability. A practical
method to implement this idea is suggested.
VL  - 80
TI  - Optimal mixing to improve the performance of batch and continuous fermentations with recombinantEscherichia coli
AV  - restricted
EP  - 370
N1  - Copyright of this article belongs to Wiley/Society of Chemical Industry.
Y1  - 2005///
PB  - wiley/Society of Chemical Industry
JF  - Journal of Chemical Technology & Biotechnology
KW  - recombinant fermentations; plasmid stability; incomplete mixing; distribution of mixing
SN  - 0268-2575
SP  - 361
ER  -